Analysis of the budding yeast pH 4–7 proteome in meiosis

Grassl, Julia, Scaife, Caitriona, Polden, Julie, Daly, Catherine N., Iacovella, Maria G., Dunn, Michael J. and Clyne, Rosemary K. (2010) Analysis of the budding yeast pH 4–7 proteome in meiosis. Proteomics, 10 (3). pp. 506-519. ISSN 1615-9853

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Abstract

Meiosis, the developmental programme generating haploid gametes from diploid precursors, requires two cell divisions and many innovations. In budding yeast, a large number of genes are expressed exclusively during meiosis while others are repressed compared to vegetative growth. Microarray analysis has shown that gene expression during meiosis is highly regulated, and has been used to classify yeast genes according to meiotic temporal expression pattern. In this study, we have begun to investigate the kinetics of meiotic protein expression using a proteomics approach. 2-D DIGE was used to characterise the temporal protein expression patterns of the budding yeast pH 4–7 proteome in meiosis. More than 1400 meiotic protein spots were visualised and at least 63 spots were temporally regulated during meiosis in a statistically significant manner. Gel spots with significant expression changes were excised and 26 unique proteins were identified using LC-MS/MS. The identified proteins could be classified into functional categories and the genes encoding a number of these were previously shown to be involved in yeast sporulation and meiosis. This data set was used to assemble the first differential 2-D PAGE map of budding yeast meiosis, which can be accessed through a web server. This work represents one of the first quantitative proteomic analyses of meiosis in yeast and will provide a valuable resource for future investigations.

Item Type: Article
Keywords (uncontrolled): 2-D DIGE; meiosis; microbiology; protein expression; proteome map; yeast
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 11112
Depositing User: Devika Mohan
Date Deposited: 02 Jul 2013 05:47
Last Modified: 13 Oct 2016 14:27
URI: https://eprints.mdx.ac.uk/id/eprint/11112

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