A novel treatment strategy for ovarian cancer based on immunization against zona pellucida protein (ZP) 3

Rahman, Nafis A. and Bennink, Herjan J. T. C. and Chrusciel, Marcin and Sharp, Victoria and Zimmerman, Yvette and Dina, Roberto and Li, Xiangdong and Ellonen, Antti and Rivero-Müller, Adolfo and Dilworth, Stephen M. and Ghaem-Maghami, Sadaf and Vainio, Olli and Huhtaniemi, Ilpo (2012) A novel treatment strategy for ovarian cancer based on immunization against zona pellucida protein (ZP) 3. The FASEB Journal, 26 (1). pp. 324-333. ISSN 0892-6638

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Abstract

We tested the principle of treating malignant ovarian tumors by vaccination against their ectopically expressed protein, zona pellucida glycoprotein (ZP) 3, using as the experimental model the granulosa cell tumors that develop in transgenic mice expressing the simian virus 40 T-antigen under the inhibin-α promoter (inhα/Tag). We found high ZP3 expression in granulosa cell tumors of the transgenic mice, in human surface ovarian cancer and granulosa cell lines, and in human granulosa cell tumors and their metastases. Early preventive immunization (between 2 and 5.5 mo of age) of transgenic mice with recombinant human (rh) ZP3 prevented ovarian tumorigenesis, and delayed therapeutic immunization (between 4.5 and 7 mo) reduced weights of existing tumors by 86 and 75%, respectively (P<0.001), compared to vehicle-treated control mice. No objective side effects of the immunizations were observed. Liver metastases were found in nontreated/vehicle-treated controls (n=7/39), but none following active rhZP3 immunizations (n=0/36; P<0.05). Immunization with rhZP3 was highly effective, as demonstrated by the induction of anti-ZP3 antibodies, as well as proliferative responses to the ZP3 antigen. These results signal rhZP3 immunization as a novel strategy to be developed for the immunotherapy of ovarian granulosa cell tumors, as well as for that of other malignancies that may express ZP3.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences
A. > School of Science and Technology > Natural Sciences > Molecular Biology group
Item ID: 11093
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Depositing User: Devika Mohan
Date Deposited: 01 Jul 2013 08:06
Last Modified: 13 Oct 2016 14:27
URI: http://eprints.mdx.ac.uk/id/eprint/11093

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