Technical note: effects of arsenate (AS5+) on growth and production of glutathione (GSH) and phytochelatins (PCS) in chlorella vulgaris

Jiang, Ying, Purchase, Diane, Jones, Huw and Garelick, Hemda (2011) Technical note: effects of arsenate (AS5+) on growth and production of glutathione (GSH) and phytochelatins (PCS) in chlorella vulgaris. International Journal of Phytoremediation, 13 (8). pp. 834-844. ISSN 1522-6514

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Abstract

The effect of arsenate (As5+) on growth and chlorophyll a production in Chlorella vulgaris, its removal by C. vulgaris and the role of glutathione (GSH) and phytochelatins (PCs) were investigated.C. vulgaris was tolerant to As5+ at up to 200 mg/L and was capable of consistently removing around 70% of the As5+ present in growth media over a wide range of exposure concentrations. Spectral analysis revealed that PCs and their arsenic-combined complexes were absent, indicating that the high bioaccumulation and tolerance to arsenic observed was not due to intracellular chelation. In contrast, GSH was found in all samples ranging from 0.8 mg/L in the control to 6.5mg/L in media containing 200 mg/L As5+ suggesting that GSH plays a more prominent role in the detoxification of As5+ in C. vulgaris than PC. At concentrations below 100 mg/L cell surface binding and other mechanisms may play the primary role in As5+ detoxification, whereas above this concentration As5+ begins to accumulate inside the algal cells and activates a number of intracellular cell defense mechanisms, such as increased production of GSH.The overall findings complement field studies which suggest C. vulgaris as an increasingly promising low cost As phytoremediation method for developing countries.

Item Type: Article
Keywords (uncontrolled): Chlorella vulgaris, arsenate accumulation, glutathione, phytochelatins
Research Areas: A. > School of Science and Technology > Natural Sciences
Item ID: 10707
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Depositing User: Josie Joyce
Date Deposited: 05 Jun 2013 13:07
Last Modified: 13 Oct 2016 14:26
URI: https://eprints.mdx.ac.uk/id/eprint/10707

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