RIP140 directs histone and DNA methylation to silence Ucp1 expression in white adipocytes

Kiskinis, Evangelos, Hallberg, Magnus, Christian, Mark, Olofsson, Martina, Dilworth, Stephen M., White, Roger and Parker, Malcolm G. (2007) RIP140 directs histone and DNA methylation to silence Ucp1 expression in white adipocytes. The EMBO Journal, 26 (23). pp. 4831-4840. ISSN 1460-2075

Full text is not in this repository.

Abstract

Nuclear receptors control the function of cells by regulating transcription from specific gene networks. The establishment and maintenance of epigenetic gene marks is fundamental to the regulation of gene transcription and the control of cell function. RIP140 is a corepressor for nuclear receptors that suppresses transcription from a broad programme of metabolic genes and thereby controls energy homoeostasis in vivo. Here we show by analysis of Ucp1, a gene which is typically expressed in brown but not white adipocytes, that RIP140 is essential for both DNA and histone methylation to maintain gene repression. RIP140 expression promotes the assembly of DNA and histone methyltransferases (HMTs) on the Ucp1 enhancer and leads to methylation of specific CpG residues and histones as judged by bisulphite genomic sequencing and chromatin immunoprecipitation assays. Our results suggest that RIP140 serves as a scaffold for both DNA and HMT activities to inhibit gene transcription by two key epigenetic repression systems.

Item Type: Article
Research Areas: A. > School of Science and Technology > Natural Sciences > Molecular Biology group
A. > School of Science and Technology > Natural Sciences
Item ID: 10100
Useful Links:
Depositing User: Professor Stephen M. Dilworth
Date Deposited: 19 Feb 2013 06:18
Last Modified: 13 Oct 2016 14:26
URI: https://eprints.mdx.ac.uk/id/eprint/10100

Actions (login required)

Edit Item Edit Item